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Heparin Reacts With and Inactivates Nitric Oxide

Whitaker Cardiovascular Institute, Evans Department of Medicine; Department of Biochemistry, Boston University School of Medicine; Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Dorinda G. Goodman, BA

Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston, MA

Scott R. Willoughby, PgD

Ying-Yi Zhang, PhD

George N. Welch, MD

Jane E. Freedman, MD

Song Ye

Catherine E. Costello, PhD

Joseph Loscalzo, MD, PhD

Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston, MA

Although heparin is a well-known anticoagulant, in some cases it promotes a prothrombotic state and does so through both antibody-dependent and antibody-independent platelet activation. In this study, heparin was found to reverse the antiplatelet effect of an NO donor, S-nitroso-glutathione (SNO-Glu), with an EC₅₀ of 1.8 U/mL. Ultraviolet/visible spectral analysis and the Griess assay showed that increasing heparin concentrations on a dose-dependent basis eliminated acidified NOx species. Since heparin is a heterogeneous mixture of glycosaminoglycans, the effects of six different heparin disaccharides were compared with various substitutions on the hexose rings to determine which functional group(s) of the polysaccharide interact with acidified NOx. Among the six disaccharides tested, only types I-S and 1I-S had the effect, suggesting that the sulfamino-group at the C2 position of the glucosamine moiety was critical for the elimination of acidified NOx species. Mass spectrometry experiments gave results consistent with these observations, indicating that only the I-S and 11-S heparin disaccharides were modified upon treatment with NaNO₂/HCl. Negative-ion electrospray ionization MS and tandem MS analyses of the native compounds and their deuteriumlabeled analogs confirmed that the reaction products from nitrosation of these N-sulfated disaccharides had eliminated the C2-sulfamino-moiety and replaced it with methoxide derived from the solvent. Participation of the 6-sulfato-substituent appears to facilitate the elimination reaction. These data show that heparin can impair the antiplatelet properties of nitric oxide by interacting with the nitrosating species, and suggest that heparin-like glycosamino-glycans may interact with endothelium-derived nitric oxide in vivo to regulate the bioactivity of this important antiplatelet and vasorelaxant substance.

Key Words: heparin • nitric oxide • disaccharides • mass spectrometry

Journal of Cardiovascular Pharmacology and Therapeutics, Vol. 6, No. 2, 163-173 (2001)
DOI: 10.1177/107424840100600208


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