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Increased Intramural Retention After Local Delivery of Molecules with Increased Binding Properties: Implications for Regional Delivery of Pharmacologic Agents

University of Pennsylvania, Cardiovascular Division, Philadelphia, Pennsylvania

Khawar Mehdi

Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana

Kevin M. Sowinski

Purdue University, School of Pharmacy and Pharmacal Sciences, Department of Pharmacy Practice, Indianapolis, Indiana

Sang Hong Baek

Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana

Keith L. March

Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana

Background: Catheter-based local vascular delivery results in concentrated quantities of pharmaceutical agents or genes into focal areas of the arterial wall. However, intramural retention is short and has reduced the potential efficacy of this approach. It was postulated that agents that possess increased intramural binding would show increased intramural reten tion. Platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF) were models of agents with increased cellular and extracellular matrix binding properties.

Methods and Results: The delivery efficiency and intramural retention of 2 mL of saline containing I¹²⁵ labeled PDGF (n = 35 arteries) and bFGF (n = 24) were compared with albumin (n = 21) after local delivery into porcine coronary arteries. Animals were sacrificed at three or more prespecified timepoints: immediately after delivery, 1 day, or 3 days after delivery and if necessary at 5 or 7 days to document prolonged retention. Autoradiograms of the arterial sections were evaluated for the extent of delivery. Delivery efficiency, defined as the amount leaving the catheter and retrieved from the arterial wall, was 0.60% ± 0.42% for albumin, 1.98% ± 0.88% for PDGF (P = .001), and 0.31% ± 0.11% for bFGF. The calculated intramural half-life of albumin was 7.4 hours, 56.2 hours for PDGF, and 14.9 hours for bFGF (P = .0001 for PDGF). Infusate covering >50% of the medial area was observed in 85% of arteries immediately after delivery. Although myocardial delivery was similar for albumin, PDGF, and bFGF, myocardial retention was significantly longer for bFGF (P < .001).

Conclusions: Molecules that exhibit preferential intramural binding show a longer intra mural residence duration than solutes without such binding properties. In addition, delivery and subsequent prolonged retention in the myocardium can be obtained by local delivery via the arterial lumen of solutions with preferential binding properties.

Key Words: local drug delivery • restenosis • atherosclerosis • gene therapy.

Journal of Cardiovascular Pharmacology and Therapeutics, Vol. 4, No. 2, 103-112 (1999)
DOI: 10.1177/107424849900400205


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