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Sodium Ferulate Modified Gene Expression Profile of Oxidized Low-Density Lipoprotein-Stimulated Human Umbilical Vein Endothelial Cells

Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, Hubei, PR China, Nanyang Medical College, Henan, PR China

Zhuoyue Bi, MD

School of Pharmacy, Wuhan University, Wuhan, PR China, wbaohua{at}hotmail.com

Yang Li, MD, PhD

Hubei Provincial Center for Disease Control and Prevention, Wuhan, PR China

Hong Zheng, MD

School of Basic Medical Science, Yunyang Medical College, Hubei, PR China

Li Li, MD

Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, Hubei, PR China

Jingping Ouyang, MD

Department of Pathology and Pathophysiology, School of Medicine, Wuhan University, Wuhan, PR China

Baohua Wang, MD, PhD

Department of Pathology and Pathophysiology, School of Medicine, Wuhan University, Wuhan, PR China

Yongyi Bi, MD, PhD

Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, Hubei, PR China

Oxidized low-density lipoprotein (ox-LDL) is known to trigger vascular injury in atherosclerosis development. Sodium ferulate is an effective component from Chinese medicines with various beneficial cardiovascular pharmacological activities. Here, we investigated the effects of sodium ferulate on the gene expression profile of ox-LDL-stimulated endothelial cells. Cultured human umbilical vein endothelial cells (HUVECs) were treated with ox-LDL (50 µg/mL) in the absence or presence of sodium ferulate (5 µmol/L). Sodium ferulate significantly reduced ox-LDL-induced endothelial cell death as evaluated by cell viability assay. Human oligonucleotide microarray analysis demonstrated that a total of 32 ox-LDL-induced genes were significantly downregulated to control levels by sodium ferulate. These genes included members from families of chemokine, inflammatory factor, growth factor, and nuclear receptor. These data provided an overview of the gene expression profile of endothelial cells in response to ox-LDL and sodium ferulate, and demonstrated that sodium ferulate could regulate the expression of inflammation-related genes in endothelial cells and has the potential to benefit endothelial function in the setting of atherosclerosis.

Key Words: sodium ferulate • oxidized low-density lipoprotein • endothelial cells • microarray analysis • atherosclerosis

This version was published on December 1, 2009

Journal of Cardiovascular Pharmacology and Therapeutics, Vol. 14, No. 4, 302-313 (2009)
DOI: 10.1177/1074248409347986


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